Composite

Part:BBa_K4180006:Design

Designed by: HuiChing Kuo   Group: iGEM22_KCIS_Xiugang_Taipei   (2022-08-28)


pGal1,10-snf1Δ2-306aa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The team used XmaI and KpnI to replace the SPT5 gene on the original plasmid


Source

snf1Δ2-306aa, N-terminus truncated from amino acid 2 to 306 deleting kinase domain of SNF1, where the phosphorylation on Thr210 activates the catalytic activity of SNF1 is amplified from yeast genomic DNA

References